Why ribonucleoprotein?

Delivering CRISPR-Cas ribonucleoprotein (RNP) complexes as opposed to plasmids has several advantages in the context of genome editing:

In summary, delivering CRISPR-Cas RNPs offers several advantages over plasmids, including increased specificity, reduced risk of integration, enhanced safety, increased efficiency, simplified experimental design, and faster results. However, it's important to carefully consider the specific requirements of the experiment, the cell type being targeted, and the desired outcomes when selecting the appropriate delivery method for CRISPR-Cas genome editing.